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首页> 外文OA文献 >Switching of Flagellar Motility in Helicobacter pylori by Reversible Length Variation of a Short Homopolymeric Sequence Repeat in fliP, a Gene Encoding a Basal Body Protein
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Switching of Flagellar Motility in Helicobacter pylori by Reversible Length Variation of a Short Homopolymeric Sequence Repeat in fliP, a Gene Encoding a Basal Body Protein

机译：通过fliP，编码基础体蛋白基因的短均聚物序列重复的可逆长度变化，切换幽门螺杆菌鞭毛运动。

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The genome of Helicobacter pylori contains numerous simple nucleotide repeats that have been proposed to have regulatory functions and to compensate for the conspicuous dearth of master regulatory pathways in this highly host-adapted bacterium. H. pylori strain 26695, whose genomic sequence was determined by The Institute for Genomic Research (TIGR), contains a repeat of nine cytidines in the fliP flagellar basal body gene that splits the open reading frame in two parts. In this work, we demonstrate that the 26695C9 strain with a split fliP gene as sequenced by TIGR was nonflagellated and nonmotile. In contrast, earlier isolates of strain 26695 selected by positive motility testing as well as pig-passaged derivatives of 26695 were all flagellated and highly motile. All of these motile strains had a C8 repeat and consequently a contiguous fliP reading frame. By screening approximately 50,000 colonies of 26695C9 for motility in soft agar, a motile revertant with a C8 repeat could be isolated, proving that the described switch is reversible. The fliP genes of 20 motile clinical H. pylori isolates from different geographic regions possessed intact fliP genes with repeats of eight cytidines or the sequence CCCCACCC in its place. Isogenic fliP mutants of a motile, C8 repeat isolate of strain 26695 were constructed by allelic exchange mutagenesis and found to be defective in flagellum biogenesis. Mutants produced only small amounts of flagellins, while the transcription of flagellin genes appeared unchanged. These results strongly suggest a unique mechanism regulating motility in H. pylori which relies on slipped-strand mispairing-mediated mutagenesis of fliP.
机译：幽门螺杆菌的基因组包含许多简单的核苷酸重复序列，这些重复序列已被提议具有调节功能并补偿在这种高度宿主适应的细菌中主要调节途径的明显缺乏。幽门螺杆菌菌株26695，其基因组序列由基因组研究所（TIGR）确定，在fliP鞭毛基体基因中包含9个胞苷的重复序列，将开放阅读框分为两个部分。在这项工作中，我们证明了具有TIFL测序的fliP分裂基因的266​​95C9菌株是无鞭毛的和不运动的。相反，通过阳性运动试验选择的菌株26695的早期分离株以及猪的26695传代的衍生物都带有鞭毛并且具有很高的运动力。所有这些运动菌株均具有C8重复序列，因此具有连续的fliP阅读框。通过在软琼脂中筛选大约50,000个菌落26695C9的运动性，可以分离出具有C8重复序列的运动型回复体，证明了所述开关是可逆的。来自不同地理区域的20个活动性幽门螺杆菌临床分离株的fliP基因具有完整的fliP基因，其中重复了8个胞苷或CCCCACCC序列。通过等位基因交换诱变构建了能动的，菌株C695的C8重复分离株的同基因fliP突变体，发现其在鞭毛生物发生中存在缺陷。突变体仅产生少量鞭毛蛋白，而鞭毛蛋白基因的转录似乎没有变化。这些结果有力地提出了一种独特的调节幽门螺杆菌运动性的机制，该机制依赖于滑动链错配对介导的fliP诱变。

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